Evolutionary Essay

Evolutionary Essay

Caryn Bradshaw

3^rd hour

The fact of evolution is the backbone of biology, and biology is thus in the peculiar position of being a science founded on an improved theory, is it then a science or faith?"

                                                -Charles Darwin

            Everyday plants and animals are growing in size and population, each living organism looks so different from another. But are they really that different? In this paper I will explore the people who have discovered and researched the theory of evolution and how they have changed the way we look at science.

           

            Evolution has been a popular subject in Biology for many years, but who really sparked the idea? Jean Baptiste De Lamarck was the first to describe a mechanism by which he concluded that evolution could not occur. This proposition was known as: ‘the inheritance of acquired characteristics”. His theory was based off of creatures that inherit different features from other creatures that are like them. For example, salamanders have short legs; therefore they cannot walk across tall grass. So suppose these salamanders started to slither on their bellies to get from place to place and since they didn’t use their legs their leg muscles wasted away and the salamanders became legless. Lamarck’s theory states that this trait was then passed on to the salamander’s off spring. Lamarck then argues that the legless salamander evolved from the salamander by inheriting the characteristic of having no legs.

           

            Charles Darwin then left off where Lamarck left off. Darwin was always fascinated by plants and animals ever since he was a young boy. He traveled around the world to observe different species of animals and started a notebook of each one he found. On his travel to the Galapagos Islands he was surprised to find that the birds their were slightly different from one another. He realized that each bird particularly the Finch was different according to the different kinds of environments. Returning from his travels he had identified thirteen species of finches that had different features in the Galapagos Islands. Each bird although they were the same species, had different beak shapes and sizes. The beak varieties were also associated with the way the diet was with certain types of foods and the different environments. He came to the conclusion that the original South American finches reached the Island then separated to different areas where each bird had to adapt to that environment and its conditions. Over many years they would change to allow them to get food, shelter, and to be able to reproduce. Darwin then came to the understanding that each organism is slightly different from one another. Each organism would need to successfully reproduce and pass on their traits to the next generation so that those traits would become more common and the population would evolve.

           

            Thomas Malthus, an English clergyman published “Essay on the Principals of Population” which spoke of human populations. Thomas Malthus stated that the human population would double every twenty-five years unless they are limited in food supply. This essay was read by Darwin and it sparked an idea that plants and animals are the same way.  These organisms’ populations could increase rapidly unless they are constantly affected by predators, diseases, or limited with food, water, or other resources. He realized that the plant or animal that is fit for survive against these things would be the species that would survive and pass on their traits to the next generation. For example, suppose you are observing brown and green beetles. You see that there is a decrease in population for the green beetles and an increase of population in the brown beetles.  You may wonder why there is such a different number of each beetle, and that is when the first mechanism of microevolution I will talk about comes into the picture.  Since the brown beetles can blend in with the dirt or the bark of a tree, they are easily hidden from birds or other predators. On the other hand the green beetles can not be hidden therefore they are eaten and are not able to pass on their traits to the next generation. The brown beetles will proceed to reproducing, as a result increasing their population and carrying on their “brown genes” this is called “natural selection”. There are times though when some of the green genes from the green beetle are randomly mutated into brown genes. This type of mechanism is very rare and can not have a big change in the frequency over one generation but it can happen in any organism therefore playing the part of “mutation” in microevolution’s mechanisms. Another reason for the decease in green beetles and the increase of brown beetles would because of where the beetles are located. If there are many trees and a lot of areas with dirt or mud you are most likely to find many brown beetles. This is where they blend in and are protected from predators. But if there are a lot of bushes, grass, and green areas you are most likely to find the green beetles for the same reason that the brown beetles are where there are matching surroundings. Over time the green beetles in your area will want to move to a place where there are more places to blend in with their surroundings. This means that “migration” will take place. Many of the green beetles could not survive in your area in which the brown beetles could so they have migrated to a place where they can blend in more to their surroundings. This could be a reason why the there has been a decrease in the population of the green beetle. Not everything in the population changes in the two types of beetles has to do with their surroundings and colors of the environment they are in. When the beetles reproduce, by some way more brown genes than green genes could end up in the off spring. Brown genes could occur slightly more frequently in the offspring therefore increasing the population of the brown beetle in your area. This process is call “genetic drift”.

            After all of this talk of what Darwin found and how species are all alike in some kind of way you may ask, where is the evidence in all of this research? Well, there is much evidence that has been researched and found throughout history. This includes similarities in DNA, embryology, and fossils. Similarities in humans and primates show that DNA plays a role in the evidence of evolution. Research shows that the jaw of an ape and a human show similar traits from the teeth and the bone structure of the jaw. Fossils also show evidence in evolution, many fossils were found in the ocean from pre-historic times to present day of many different fish. In the fossils from pre-historic times the fish are much bigger, but compared to present day fossils the fish are basically the same except for the size. The way both fishes are formed by the way it looks in the skeleton it left behind you can see that both fish have the same form of skeleton and the same shape. Embryology plays a big role when it comes to evidence in evolution. Comparing a chicken embryo and a human embryo is a great example when finding evidence for evolution. When you put a picture of a chicken embryo next to a human embryo you can see that each one has the same shape and form of one another’s. Both the chicken and the human have Pharyngeal pouches and they both also have a post-anal tail located in the exact same spot.

            In my opinion, Darwin’s evidence about species changing over time is the most convincing for me. Although some people do not agree with some of the ideas he has sparked, I feel that with his research he has concluded some of the most changes in animals and plants. His “Survival of the fittest” theory states that the animal and plant that is best fitted to survive in the environment it is put in is the specie that is most likely to survive and be able to pass on their traits to the next generation. His research on the finches he had found in different areas in the Galapagos Islands prove that animals can change either over time to either survive in their environment or traits from the generation before them had changed. Fossils are also great evidence that species have changed over time because we still have that evidence today. Looking at the fish fossils and comparing them to present day ones you can see the similarities but also the differences that they have changed over time. Looking at the bone structure and how it is formed you could see that it is the same. The differences in these fish were the size in teeth and the fish’s size in general. In the pre-historic days there were many different predators in the sea at that time. So the fish needed to be bigger in size as well as teeth to defend itself from these predators. Today, there are still predators in the sea after fish but there aren’t as many. Therefore the size of teeth and the general size are much smaller. From looking at fossils all of these observations are enough evidence that species have changed over time.

            The fact of evolution is the backbone of biology, and biology is thus in the peculiar position of being a science founded on an improved theory, is it then a science or faith?"

                                                -Charles Darwin

            I use this quote again to conclude my paper because Darwin asks a very complex question that can’t be answered in a day. Is evolution in fact a science or a faith? Well Darwin did get a lot of criticism for his theories and his research in science. Some people took it as though Darwin was trying to replace God, but in all reality he was only doing something he loved to do and that he was good at. It just happened that he had stirred up a whole whir wind of mind boggling questions and intelligent research as well as theories. So, it depends on what you think of Mr. Darwin, and how you perceive the world and the organisms that happen to live on this planet. Is evolution science or is it a faith?

                                                            Bibliography

     

Title: Charles Darwin quotes

Site: http://www.darwin-literature.com/l_quotes.html

Evolutionary Essay

Caryn Bradshaw

 Biology

3^rd hour

Evolution Essay

“Change is at the very core of evolution and without it, all creatures would look alike and behave the same way.” -Martin Dansky-

          Things are growing and changing around us everyday. Creatures big and small all over the world posses their own unique characteristics, but when you get to the bottom of things, every living, breathing, organism is made up in the same way.  So you would ask, if we are all made up the same way, then how are we so diverse?  There is no simple answer, but there is a theory.  This theory has grown over the years and is still studied today; it is the Theory of Evolution.  The definition of evolution is a change in the lines of decent of living creatures over time.  This means that it is believed new species develop from earlier species as a result of molecular changes.  Now, this theory is commonly perceived as scientists trying to explain the origin of life.  However, it is simply an explanation as to how organisms formed after life was created.  In addition, evolution is not something that just occurred in the past.  It is happening right at this moment and will continue to happen for eternity!  Included in this paper is information on the early theories of evolution, examples of microevolution occurring, and evidence of the theory of macroevolution.  Also included will be my personal opinion on the most convincing evidence of evolution.

            In the 18^th century, comparative morphology became a study among scientists.  Comparative morphology is the systematic study of similarities and differences in body plans between major groups.  Studying this requires taking a closer look at bone structures and physical appearances of life forms.  This study brought about a question in scientists minds.  How can animals appear different in bodily features, but so much alike in others?  An example of a study performed to look at comparative morphology would be to compare the pelvic structure of a manatee.  Now, for a sea manatee, there would be no need for a pelvic structure because they don’t walk.  However, scientists have found that sea manatees do in fact have a ghost socket where a pelvic structure would be found.  Why is this?  There were intermediate fossils found that gave evidence to there being such a thing as a “land manatee.”  These fossils contained many of the same characteristics of sea manatees, but included pelvic structure meaning they were able to walk.  How is this possible?

            A well-respected anatomist, Georges Cuvier, was first to come up with the theory of Catastrophism.  This theory suggested that at one point in time, there was a universal catastrophe that destroyed many of the life forms on the planet.  Then the survivors repopulated the world.  Cuvier stated that the offspring of those species were not new, but were just ones that scientists had not found fossils of yet dating clear back to the time of creation. 

            Some people were able to accept Cuvier’s theory, but others, like a man named Jean Lamarck, set out to prove the theory wrong.  He knew that inheritance of characteristics had something to do with the way species grew and changed.  So he hypothesized that environmental pressures and needs bring about permanent changes in animals and their offspring.  For example, if a species of giraffes have a short neck and are unable to reach high branches for food, they simply stretch out their necks to reach it.  By stretching out their necks day after day for food, the giraffe develops a long neck.  Once the long neck is developed, the giraffe passes the characteristic on to its offspring.  Although Lamarck’s theory is not necessarily reasonable, he was right with the fact that the environment plays a role in evolution.

            In the 1800’s, a geologist by the name of Charles Lyell began contradicting Lamarck’s theories.  He spent his time examining sedimentary rock layers and their age.  While many scientists suggested the earth was about 6,000 years old, Lyell came to the conclusion that the earth had to be millions of years old.  He came about this conclusion because there were so many rock layers that is was impossible for them to all form in the short period of time of 6,000 years.  This became known as the Theory of Uniformity.  After taking an even closer look at the rock layers, it was also understood that the possibility that a catastrophe occurred was nearly impossible. 

            In the middle of all of these forming theories about evolution came a man who would truly influence the way everyone viewed evolution.  In 1831, Charles Darwin embarked on a voyage across the sea to South America where he studied the diverse life on all the different islands.  Marine life was also part of his collection. 

            When Darwin returned to England, he began to review his notes and drawings of his findings.  He noticed that he had discovered some fossils of extinct animals, but then saw that some of the new species he examined resembled the extinct animals!  As he looked through his drawings and notes, he observed that not all of the species were alike, but they did show variation in size, color, and many other traits.  By reviewing all of his findings, Darwin was able to combine his new theories with those of Lyell and other scientists.  He was then able to formulate a theory that included all of the evidence found.  Variation became a huge part of Darwin’s conclusion because variation would enable the species to survive and reproduce in certain environments.  In other words, he proposed that an environment would “select” specific characteristics for a species to possess.  This is now called natural selection.  For example, if a finch lives on an island where their only food source is hard-shelled seeds, they must have a strong beak to crack the seeds open.  However, there are a few finches that lack a strong beak and have a lesser chance of survival.  Therefore, the strong beaked finches have a better chance of surviving and reproducing.  Over time, the strong beaked finches become abundant and the population of finches will be mostly strong beaked finches.  With that information you can conclude that a population is evolving if inheritable traits continue to change over time.

            Since Darwin’s theory emerged, variations in traits have been shown to be controlled by genes in DNA.  Microevolution occurs when little changes in allele frequencies are brought about by mutation of genes, genetic drift, migration, and natural selection.  When mutation in genes occurs, the result is a change in the DNA structure, which is heritable.  This occurrence is the source for diversity in species and organisms.  Gene flow and genetic drift is also a contribution to the diversity of things, but is can only cause a change in existing allele frequencies.  When alleles leave or enter a population, as a result of immigration or emigration, it is called gene flow.  This idea is much like genetic drift, however, genetic drift is brought about by chance only.  This size of a population affects the diversity of genetic drift because of the fact that small populations are more likely to loose alleles completely.

            An example of microevolution could be the guppy computer activity we did in class.  Imagine that there are all kinds of different colored guppies; some bright, some semi-bright, and some dull.  They all live in the same pond and share the same predators.  Because the brightly colored guppies attract the predators, they are eaten and unable to reproduce further.  However, the dull colored fish and even a few semi-bright guppies are less attractive and don’t get eaten as much, so they go on to survive and the population of them increases.  Now in another pond, the guppies share their habitat with fewer predators.  Since female guppies prefer to mate with brightly colored guppies, the dull colored ones are unable to mate enough to keep their population up.  So in the second pond, unlike the first pond, the brightly colored guppies make up most of the population.  As a conclusion, you can see how microevolution plays into the survival of different species.  The simplest factor in a population can play the biggest role in how an environment thrives. 

             Macroevolution is large-scale patterns, trends, and rates at a broader view.  Some examples of this broader study are fossil records, comparative morphology, and biochemistry.  However, most would say the most recognizable forms of evidence of macroevolution would be fossils.  Scientists use sedimentary rock sequences as geological time scales to define eras and eons.  Comparative morphology uses fossils as well as existing life to form similarities that show evolutionary relationships among groups.  Taking it a step further would be the use of biochemistry.  This is where scientists identify differences and similarities between species on a molecular level.  They can look at how one strand of DNA pairs with another strand from a different species and form an idea of the evolutionary distance between the two tested. 

            Out of everything I have discussed, I feel that the most convincing evidence is the study of macroevolution.  Those scientists are able to look at geological evidence, morphology, and DNA structures.  All of these things put together in my opinion are the keys to life and evolution.  You are able to look at time and age first, then you can see different structures of species and compare them to modern day species, and to take it a step further, you are able to look at DNA structures between species.  As Martin Dansky stated, “Change is at the very core of evolution and without it, all creatures would look alike and behave the same way.”  I feel this quote sums up my discussion on evolution.  We are all basically the same, and therefore we must have come from one common ancestor.  Everything in life has a cause; it’s just a matter of finding that definite reason for existence. 

Biology Video... Advice for the future!!!

Bacterial ID Lab questions

Bacterial ID Lab

Completing this virtual lab and answering the following questions helps meet Standard 3: Lab Skills.

First, go to:

http://www.hhmi.org/biointeractive/vlabs/bacterial_id/index.html

(works best in FIrefox, not Safari browser)

In this virtual lab you will assume the role of a lab technician in a modern molecular biology laboratory.  As such, you are responsible for providing lab results to medical doctors for use in diagnosing their patients.  Be sure to follow the steps of the procedure in order and to make use of the notes on the right side of the computer screen.  As you work through the lab, answer the following questions:

As the medical technician in charge of this investigation, what are you trying to determine about the tissue sample provided to you?

 I am trying to determine whether the new sequence bears a significant degree of similarity (or homology) to another known sequence.

How did you prepare the DNA to be used in this investigation?

We prepared negative and positive control reactions. Instead of the sample DNA, the positive control reaction contains positive control DNA.

Describe how PCR is used to make copies of DNA sequences. Use the animation and notebook entries in the PCR Amplification step to guide your answer. Note that you may replay the animation as needed.

Double-stranded DNA is “unzipped” with an enzyme to start the process. In PCR, single-stranded DNA is made by heating up a fragment to a certain temperature. Then it is cooled down so that the primers bind to the original DNA strands. Repeating this process over again many times you will end up with millions of DNA strands..

Summarize the technique used to purify the PCR product.

Once the PCR product is in the gel, you can cut out the stuff corresponding to the PCR product and isolate the DNA from the gel. Microfilters to filter the DNA from the PCR tube without running a gel. Microfilters can filter out all he of unneeded things in the PCR and it becomes purified.

What is produced during the sequencing prep PCR run? Use the animation and notebook as needed in thinking through your answer.

16s rDNA, is produced during the sequencing prep in the PCR run.

Describe how the automatic sequencer determines the sequences of the PCR products.

The automatic sequencer determines the sequence of the PCR by a method called Gel electrophoresis this is to separate molecules based on differences in size. The sequencer used in this lab has a thin capillary tube attached at one end to a syringe mechanism that contains a buffer solution. The tube is filled with the buffer solution and the other end inserted into one of the tubes containing the DNA pieces. Then, an electric current is applied so that the end of the tube in contact with the DNA has a negative charge and the syringe end a positive charge. Since DNA molecules are negatively charged, they start to move through the tube toward the positively charged syringe end, with the smaller pieces moving faster than the larger ones. Near the syringe end, the capillary tube passes through a laser beam that excites the fluorescent markers, and optical detectors detect the color of the fluorescence.

What does BLAST stand for?

Basic Local Alignment Search Tool

What conclusions did you make using the results of the BLAST search? Did these conclusions support a clinical diagnosis for the patient (what disease did they have)?

Using the BLAST search I figured that the DNA samples match exactly and that the patient did have a disease.

Practice Gel Trays

 Today in Biology we practiced on loading gel trays with different colored dyes. We used a micro-pipet which held 40 uls to load the trays. Loading the trays in different colors, helped us to see the different types of DNA scientists use.

 My group used different colors each time so we didn't have all of the same color for each section. As you can see to the right each color is a solid now.

After we had used the pipet we had to keep changing the plastic that held the coloring so that there wouldn't be any mistake of mixing the colors up.

 Each row is a solid and it will remain so until we plug in the electricity. With the electricity the colors will determine which ones are negative and positive energy.

As you can see to the left there is the machine that held the electricity. As i checked back with my group, we noticed that each color was going either the same direction or the opposite. The colors going to the right meant that the DNA was negative. And the DNA going the opposite direction mean that it was positive.

DNA Sequencing Graph

Conclusion: As you can see the graph above Abby had only one base change or point mutation. I think that Abby does not have the disease since her % is so high and it very close to the "norm" bases. Bob has the same percentage as Abby he also has one point mutation but his is in a different base which instead of the potion being LYS it is STOP this is called truncation mutation. I also think that Bob does not have a disease because his bases are very similar to the "norm" bases. On the other hand Carol has more than half of her bases that are different from the rest of the groups. As you can see on the graph she is a 78%. In my opinion she is the one with the disease because she is different from the group!

DNA LAB

A couple days we as a class did a wheat germ lab to extract DNA from wheat germ. These are some of my observations. When my group added the ingredients the "wheat germ soup" looked slimy and the grain floated to the bottom and some mixed in. When we added the detergent the color of the mixer turned into a yellow color. We also noticed that the ingredients got thinker as we stirred more. When the alcohol was added we observed that the wheat detergent and alcohol separated as liquids. With a few minutes of waiting we noticed that the DNA started to form in between the alcohol and wheat detergent. With it's white color and slowly forming foam, within a few more minutes stringy DNA formed. As the picture to your left shows, the DNA grew bigger!







To your right you can see a picture of the glob of DNA on the wooden stir stick. It almost looks like a form of mucus only it has a white texture and a much slimier texture. Doing this lab helped me to understand how DNA looks. I also learned how it is formed! It was interesting to create DNA and i'm glad I took something from this lab!

G-reg and Olga's Pedigree

                             Olga's

                                  Greg's

Eugenics Paper

     When I looked up the definition for eugenics it said: "the applied science or the biosocial movement which advocates the use of practices aimed at improving the genetic composition of a population". Reading this definition over I had to think that it was wrong. Eugenics was the process of elimination in the 1800's all the way to the 1960's. This experiment opened a whole new way of science, it changed everything in good and bad ways.

    The social origins of eugenics started during the Civil War and around the time of economic and social struggle. The American industry  grew as well as cities and most importantly immigration. There was an increase amount of immigration after World War I. With declining birth rates in the wealthy and powerful class industrialization demanded a change from Laissez-Fair to manage capitalism. Therefore progressivism was created. Progressive reformers grew a strong faith in science and discovery in this new science brought  discovery of eugenics.Genetics became the explanation for al of people's problems, such as pauperism, feeble-mindedness, alcoholism, rebelliousness, nomadism, and many more. Eugenics argued that their society was affected by letting these individuals be supported by the state. New immigrants were seen as a problem and heir genes were blamed for it. This led to immigration restriction. Being a way as to solve all of these problems eugenics was used as the cover of science to blame different people for their problems.

     Scientific origins of eugenics started in the 20th century as well. Only then the concerns of the environment influenced the idea that ill health, early death, insanity, and defected offspring were because of a bad environment. Thus leading to the degeneracy theory in the 1700's. With this theory the act of masturbation or "onanism" was presented as the first cause of degeneracy. Harry Sharp, a prison physician decided to perform experiments on prisoners in 1899. Sharp and his colleagues made a law in Indiana for the sterilization of degenerates. This act was the first sterilization law in the U.S.

       Research methods became a huge source at this time. Research became an effort to apply the findings of Mendel's laws to human traits. Family tree's and pedigrees were researched to find basic patterns in families. It was said by Mendel that "factors" or genes is the key to visible traits in a person. Dominant and recessive genes came into the picture from research from Mendel. Dominant genes being when there is a dominant trait expressed from a gene being inherited from one parent and a recessive gene from another. Although many traits were found, it was difficult to score traits since eugenics attempted to measure complex traits. Today we use DNA makers to follow trait inheritance which makes it easier for families to trace back in time.

Source= http://www.eugenicsarchive.org/eugenics/

Babies :) and Genes

Since we have been learning about reproduction and how there are different ways to make a baby these days I thought i would explain a few terms in making a baby! The first term I have learned would be the word: Homozygous. This word sounds like a character out of a Dr. Seuss book but really it means 2 of the same copy of a gene. For example AA, or aa. The opposite of Homozygous is another term Heterozygous which means two different copies of a gene. And an example of this would be Aa or Bb. Dominant and Recessive also play a big role in genetics. A dominant gene is a gene that shows up in a trait. A recessive gene is a hidden gene in a trait. Phenotype is a visible trait in the child for example blue eyes or big ears. A genotype are the genes that make the phenotype the genes that are present. Knowing these terms help so much when we are progressing into the baby making process!!!!

I made a baby TODAY!!!!

Last week we were paired up into groups of two of either a a male/ female group or same sex group. We both filled out a "Create-a-baby" table by flipping a coin for parent 1 and parent 2's genotype. Thus then figuring out what traits the baby would have from "mom" and "dad". As a class we learned that XX represents the females traits and XY represents the male. Having fun with this on Friday, we go together the following Monday to draw our baby. My partner Derek and I were separated to do this so that we could experiment with what we thought our baby would look like. Unfortunately his version of our son looked like an ogre and mine looked like a mamas boy! But i had fun and I learned a lot about how a child gets most of it's traits from the mom and dad!!!!

What I wish I learned in High school

I have been stressed about graduation since the first day of my senior year. I have a pretty good idea about what I want to go to college for, but then again it has changed about a million times in the last two months. Just recently I have finished my college applications and essays and although I sigh with relief, the stress of waiting to see what college I get into following the stresses of college is getting to me. The day Mr. Ludwig assigned us to read this article written by a man that was supposed to read it to a high school but had gotten kicked out for some reason, I started reading it right away. It talks about how you don't have to decide who you are going to be in the world by the end of high school. People always ask you what your major will be or what are you going into, but the truth is is that they only ask you this to start up a conversation. We are raised in the society to follow rules or follow what "the big man" says. But really college is a place to find yourself; to get to know who you are and what you want to do with your life. There is no time limit to figure this stuff out, you could figure it out the first day as a freshman in college or on your college graduation day. It doesn't matter as long as you are happy with yourself and feel confident to go out not the world and do what you want to do. This article sort of decreased my stress level and made me realize that life has no schedule to follow it's all up to you!

Onion root lab

This Onion root lab really helped me not only to understand the types of phases that are in mitosis but also to understand how to identify them. I thought that this would be a complicated task on my part because I didn't think that I could identify a tiny cell and be able to compare it to another tiny cell. This lab helped me to realize that everything in this world will never be the exact same. With the tiny cells we identified yesterday each cell of the onion root had it's own little detail and process. Interphase is a bunched up cell then the process moves on to prophase which is still a bunched up cell but the nucleus is very clear and shows p as a black dot usually in the middle of the cell or close to it. Then Metaphase comes next in the process and you can identify it as the cell slowly separating, then moving on to anaphase where there is a defined divide in the cell. The last stage is Telophase which you can identify the cell because it is divided into two separate parts and looks like halves of egg shells. Learning about the process of mitosis will probably be my favorite part of biology because I feel that even after high school I will remember what I learned about it and how to identify which stage of mitosis is which!

Mitosis lab

1.What percent of cells were in interphase?

55%

2. What percent were in mitosis?

100%

3. Which phase of mitosis takes the longest?

Interphase

4. During which stage is the nucleolus visible as a dark spot?

Interphase

5. How can you recognize a cell in metaphase?  By the two squiggly lines opposite of each other.