Tuesday, March 29, 2011

DNA Sequencing Graph


Conclusion: As you can see the graph above Abby had only one base change or point mutation. I think that Abby does not have the disease since her % is so high and it very close to the "norm" bases. Bob has the same percentage as Abby he also has one point mutation but his is in a different base which instead of the potion being LYS it is STOP this is called truncation mutation. I also think that Bob does not have a disease because his bases are very similar to the "norm" bases. On the other hand Carol has more than half of her bases that are different from the rest of the groups. As you can see on the graph she is a 78%. In my opinion she is the one with the disease because she is different from the group!

Friday, March 4, 2011

DNA LAB

A couple days we as a class did a wheat germ lab to extract DNA from wheat germ. These are some of my observations. When my group added the ingredients the "wheat germ soup" looked slimy and the grain floated to the bottom and some mixed in. When we added the detergent the color of the mixer turned into a yellow color. We also noticed that the ingredients got thinker as we stirred more. When the alcohol was added we observed that the wheat detergent and alcohol separated as liquids. With a few minutes of waiting we noticed that the DNA started to form in between the alcohol and wheat detergent. With it's white color and slowly forming foam, within a few more minutes stringy DNA formed. As the picture to your left shows, the DNA grew bigger!







To your right you can see a picture of the glob of DNA on the wooden stir stick. It almost looks like a form of mucus only it has a white texture and a much slimier texture. Doing this lab helped me to understand how DNA looks. I also learned how it is formed! It was interesting to create DNA and i'm glad I took something from this lab!